Enzymes and Inhibitors
MilliporeSigma™ Calbiochem™ Y-27632
Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
Thermo Scientific Chemicals Deoxyribonuclease I, bovine pancreas, Purified
Deoxyribonuclease I, CAS # 9003-98-9, also known as DNase I, is an endonuclease isolated from bovine pancreas used mainly to remove deoxyribonucleic acid (DNA) from protein samples.
Thermo Scientific™ FastDigest MbiI
Cut at CCGCTC(-3/-3)^ sites with Thermo Scientific™ FastDigest™ BsrBI (MbiI), which performs best using universal FastDigest Buffer (Isoschizomers: AccBSI).
O-Nitrophenyl-β-D-Galactopyranoside, MP Biomedicals™
Useful in the detection of lacZ activity. O-Nitrophenyl-β-D-Galactopyranoside, MP Biomedicals is a colorimetric substrate for b-galactosidase and a cell culture component for molecular genetics. ONPG reactions are rapid and occur within a 24-hour per
Thermo Scientific™ BseXI Buffer (10X)
Optimize digestions with BseXI restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ BamHI-Lsp1109I Buffer (10X)
Optimize digestions with BamHI and Lsp1109I restriction enzymes with this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ MvaI (BstNI) (10 U/μL)
Cut at CC^WGG sites with MvaI (BstNI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI).
Thermo Scientific™ FastDigest BfmI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest HinfI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest Hpy8I
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest XceI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Nuclease P1, MP Biomedicals™
Exhibits both phosphodiesterase and monoesterase activities. Nuclease P1, MP Biomedicals is a single strand-specific nuclease that exhibits high selectivity for single-stranded nucleic acids and single-stranded regions in double-stranded nucleic acids. De
Thermo Scientific™ Thermus thermophilus DNA Ligase
Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.
Thermo Scientific™ PvuII (10 U/μL)
The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.